Construction of hiPS-based drug evaluation system for Alzheimer's disease

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14962
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Kyoto University
• Principal Investigator: Akaike Akinori 京都大学, 薬学研究科, 客員教授 (80135558)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ヒトiPS由来細胞 / アルツハイマー病 / 薬効評価

Outline of Final Research Achievements

We investigated whether the mechanism of Aβ-induced neurotoxicity accumulated in rat cultured neurons applied to human neurons and aimed to construct human iPS-based drug evaluation system for Alzheimer's disease. First, iPS cells were differentiated into neural stem cells (NSCs), and we obtained hiPS-differentiated NSCs which were not requiring feeder cells. Next, we examined that effect of neuron-astrocyte interaction on cytotoxicity using rat neuron monoculture and rat neuron-astrocyte mixed culture. It is suggested that the interaction between neuron and astrocyte reduce the susceptibility to Aβ-induced neurotoxicity.

Free Research Field

薬理学