2016 Fiscal Year Final Research Report
Construction of hiPS-based drug evaluation system for Alzheimer's disease
Project/Area Number |
15K14962
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Pharmacology in pharmacy
|
Research Institution | Kyoto University |
Principal Investigator |
Akaike Akinori 京都大学, 薬学研究科, 客員教授 (80135558)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | ヒトiPS由来細胞 / アルツハイマー病 / 薬効評価 |
Outline of Final Research Achievements |
We investigated whether the mechanism of Aβ-induced neurotoxicity accumulated in rat cultured neurons applied to human neurons and aimed to construct human iPS-based drug evaluation system for Alzheimer's disease. First, iPS cells were differentiated into neural stem cells (NSCs), and we obtained hiPS-differentiated NSCs which were not requiring feeder cells. Next, we examined that effect of neuron-astrocyte interaction on cytotoxicity using rat neuron monoculture and rat neuron-astrocyte mixed culture. It is suggested that the interaction between neuron and astrocyte reduce the susceptibility to Aβ-induced neurotoxicity.
|
Free Research Field |
薬理学
|