2016 Fiscal Year Final Research Report
Use of Fungal Strains Resistant to Ribosome Targeting-Antibiotics for Discovery of Cryptic Secondary Metabolites
| Project/Area Number |
15K14967
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 糸状菌 / 二次代謝物 / 休眠遺伝子 / リボソーム工学 |
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| Outline of Final Research Achievements |
The potential of filamentous fungi as a rich source of natural products has been unveiled by genome sequencing studies that have shown the presence of many cryptic biosynthetic gene clusters. The access for secondary metabolites hidden in the cryptic biosynthetic pathway would increase the opportunity to discover new drug candidates. In this study, to develop a new method to stimulate cryptic biosynthetic pathway in fungi, we introduced “ribosome engineering of bacteria” into fungal secondary metabolism.
We obtained mutant strains with hygromycin B resistance from various fungi by mutagenetic approach using MNNG as a chemical mutagen. The evaluation of the secondary metabolite productivities of the mutant strains indicated that introduction of hygromycin B resistance into fungi is available to activate fungal metabolite productions. Application of the method to Chaetomium and Cordyceps fungi allowed us to isolate novel compounds whose production were silent in their wild type strains.
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| Free Research Field |
天然物化学
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