Imaging of live cells and tissues by atmospheric scanning electron microscopy

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15012
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Niigata University
• Principal Investigator: USHIKI TATSUO 新潟大学, 医歯学系, 教授 (40184999)
• Co-Investigator(Kenkyū-buntansha): 水谷 祐輔 新潟大学, 医歯学系, 助教 (40646238) ; 中島 真人 新潟大学, 医歯学総合病院, 助教 (60588250)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 走査電子顕微鏡 / 細胞と組織 / ライブイメージング / 大気圧

Outline of Final Research Achievements

We investigated the possibility of observing live cells and tissues by atmospheric scanning electron microscopy (atmospheric SEM). Our study revealed that this technique is powerful for observing plant tissues including leaf surfaces, but has some difficulty for visualizing the surface of animal cells and tissues due to several reasons such as surface unevenness, low signal intensity and the presence of surface water . To improve the quality of cell observation by　atmospheric SEM, preparation techniques including the ion liquid treatment were tested in this study.

Free Research Field

顕微解剖学