Verification of genomic diversity required for tumorigenesis

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K15024
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA KEIKO 東北大学, 医学(系)研究科(研究院), 教授 (60294972)
• Co-Investigator(Kenkyū-buntansha): FUNAYAMA RYO 東北大学, 大学院医学系研究科, 助教 (20452295) ; NAKAGAWA TADASHI 東北大学, 大学院医学系研究科, 助教 (30707013) ; HOSOGANE MASAKI 東北大学, 大学院医学系研究科, 助手 (30734347)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: 腫瘍形成 / CRISPR/Cas9 / 次世代シークエンサー / 乳癌細胞株 / 免疫不全マウス

Outline of Final Research Achievements

It has been demonstrated that solid tumors are composed of various genomic mutated cells by genomic sequencing and analysis of diversity. In this program, we tried to identify critical mutations to generate solid tumors by random mutagenesis using CRISPR/Cas9 library. We used library which randomly made deletions at promoter regions of almost all reported human genes. We infected CRISPR/Cas9 library to human cancer cell lines to generate randomly mutated cell pool. Diversity of library extracted cell line were reduced within several days culture after introduction of library compared to that of original library. This observation suggest that in introduction of library make some clones die or proliferate less, as these clones have disadvantage to survival in vitro compared to other clones.

Free Research Field

総合生物