Development of IP3 clamp method

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15027
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: The University of Electro-Communications
• Principal Investigator: Shirakawa Hideki 電気通信大学, 大学院情報理工学研究科, 准教授 (40241070)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: カルシウムシグナリング / オプトジェネティクス

Outline of Final Research Achievements

Protein-based molecular tools were developed, aiming to establish the IP3 clamp method, a novel technique to control the intracellular IP3 concentrations in living cells by light. It was shown that the combination of human phospholipase C and plant photoreceptor proteins could give the photoactivatability to an IP3-producing enzyme. We also developed fluorescent IP3 probes that have favorable excitation/emission wavelengths to be used with photoactivatable enzymes in the IP3 clamp system.

Free Research Field

分子細胞生物学