Elucidation of the molecular components and regulatory mechanism of anion channels controlling the cell homeostasis and death

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15029
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: University of Toyama
• Principal Investigator: SAKAI Hideki 富山大学, 大学院医学薬学研究部(薬学), 教授 (60242509)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 生理学 / 細胞・組織 / イオンチャネル / 生体膜 / 細胞容積

Outline of Final Research Achievements

Volume-sensitive outward rectifying (VSOR) anion channel contributes to keep cell homeostasis and to trigger cell death such as apoptosis. In this study, we tried to elucidate the molecular components of the VSOR anion channel by using cellular physiological and molecular physiological techniques.
Here, we found that SSNK-1 (tentative name) is one of essential components of the VSOR channel. SSNK-1 may be associated with LRRC8A in the membrane microdomain of plasma membrane, where they elicit the channel activity for the cell volume regulation. In contrast, SSNK-2 and SSNK-3 were not involved in formation of the VSOR anion channel.

Free Research Field

細胞生理学、分子生理学