2015 Fiscal Year Final Research Report
Purification of intestinal stem cells on the basis of their cell cycle status and its application to regenerative medicine
| Project/Area Number |
15K15032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Keywords | 腸管幹細胞 / +4幹細胞 / CBC幹細胞 / 静止期 / CDKインヒビター / p57 / Bmi1 / Lgr5 |
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| Outline of Final Research Achievements |
Identification of intestinal stem cells (ISCs), which give rise to differentiated cells in the intestine, is critical to understand mechanisms of morphogenesis and maintenance of this organ. Although two kinds of cells, referred to as CBC stem cells and +4 stem cells, were reported as ISCs, it is still presumed that more undifferentiated stem cells might exist. In this grant, we aim to purify ISCs based on the finding that tissue stem cells remain in the quiescent state of the cell cycle. We found that CDK inhibitor p57 is abundantly expressed in +4 stem cells and required for the maintenance of their quiescence. To further purify ISCs on the basis of p57 expression, we generated mice for lineage tracing experiments of p57-expressing cells in the intestine.
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| Free Research Field |
幹細胞生物学
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