Is nitiric oxide beneficial or harmful for infection of Orientia tsutsugamushi?

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15092
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Ogawa Motohiko 国立感染症研究所, ウイルス第一部, 主任研究官 (10322710)
• Co-Investigator(Kenkyū-buntansha): 佐藤 正明 国立感染症研究所, ウイルス第一部, 主任研究官 (30442966)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: つつが虫病リケッチア / マクロファージ / 活性化 / 一酸化窒素 / 増殖促進効果

Outline of Final Research Achievements

Growth of Orientia tsutsugamushi (OTS) was promoted in mouse macrophage cell line, Raw 264.7 by treatment with lipopolysaccharide (LPS) and addition of nitric oxide (NO) producing agent, NOR 5. In macrophages, it was shown that NO, which should mainly play a role of bacteria-killing, is conversely involved in the promotion of OTS-growth. By the microarray, after LPS and NOR 5 treatment, we succeeded in selecting candidate genes related to the promotion of OTS-growth out of the genes having the same expression pattern. In this study, we had a great achievement to identify the candidate genes which possibly promote the growth of OTS for further analysis.

Free Research Field

リケッチア学