Development of a cell preparation method for CNV analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Yamaguchi University
• Principal Investigator: SASAKI Kohsuke 山口大学, その他部局等, 名誉教授 (80116722)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: がん発症感受性 / コピー数多型 / 細胞遺伝学 / FISH / 散発性がん

Outline of Final Research Achievements

CNVs (copy number variations) have recently drawn attention as a reliable marker of cancer susceptibility. Indeed, it has been accumulated as evidence that certain CNVs affect cancer susceptibility in individuals. Different CNVs are associated with different cancers and at present, CNVs associated with cancer susceptibility are successively reported. However, simple and inexpensive methods for analyzing CNVs in individuals are not yet available. In this project, we tried to develop a new interphase cytogenetic method to meet these requests. An analysis of CNVs in individuals becomes very simple and easy if CNV signals in FISH (fluorescence in situ hybridization) are larger and brighter than those in conventional FISH preparations. The long treatment with formamide followed by treatment with pepsin made FISH signals large and bright. This method can be applied to practical use for estimation of cancer susceptibility in individuals.

Free Research Field

病理学