Construction of a LPS-induced ARDS pig model and multiple assay of the model.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15112
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Rakuno Gakuen University
• Principal Investigator: Miyasho Taku 酪農学園大学, 獣医学群, 講師 (50568996)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Kazuto 酪農学園大学, 獣医学群, 教授 (60244844) ; SAKAMOTO Yuichiro 佐賀大学, 医学部, 教授 (20366678) ; IMAHASE Hisashi 佐賀大学, 医学部, 助教 (10724057) ; SANO Tadashi 酪農学園大学, 獣医学群, 准教授 (50365194) ; MIYOSHI Kenjirou 酪農学園大学, 獣医学群, 講師 (90588138) ; OKAMOTO Minoru 酪農学園大学, 獣医学群, 准教授 (60372877)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 動物モデル / LPS / 敗血症 / 肺障害 / モデル構築 / モニター / メディエーター

Outline of Final Research Achievements

We attempted to construct a LPS-induced ARDS pig model. First, LPS was continuously administered at 160μg/kg/hr for 75 minutes, and then LPS was continuously administered at 80μg/kg/hr until it was confirmed that the P/F ratio < 300 continued for more than 30 minutes. As a result, a LPS-induced ARDS pig model was generated in about 120 minutes.
Perfusion index measured with noninvasive pulse oximeter and pulmonary vascular permeability index measured by invasive hemodynamic monitor were reacted more sensitively than blood pressure and heart rate which are general indicators of shock, and it also were reflected its prognosis sharply. In all of the ARDS group, blood HMGB1 concentration was highest immediately before death.

Free Research Field

生化学