Elucidation of erythroid differentiation using fsn mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15117
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Research Institute, Shiga Medical Center
• Principal Investigator: Kinoshita Kazuo 滋賀県立成人病センター(研究所), 遺伝子研究部門, 専門研究員 (50293874)
• Co-Investigator(Renkei-kenkyūsha): UEMURA Munehiro 滋賀県立成人病センター研究所, 主任主査 (30568390)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 赤血球 / 脱核

Outline of Final Research Achievements

Developing erythrocytes undergo a process of enucleation, which expells the nucleus out of the cell. To overcome a problem of inefficient enucleation of erythroblasts under the culture conditon, which is a major hurdle against establishing an industry-scale erythrocyte production, elucidation of molecular mechanism of enucleation may be required. We performed experiments to analyze function of Ttc7 gene, which is the causative gene of flaky skin (fsn) mice, a mutant strain with abnormal erythrocyte differentiation and enucleation. We found that erythroid differentiation is abnormal in the bone marrow of fsn mice. We could isolate and maintain extensively self-renewing erythroblasts (ESRE) from the fetal liver of fsn mice, which could enucleate under culture as efficiently as the wild-type ESRE. We identified proteins that bind with Ttc7 protein.

Free Research Field

生化学