The biomarker candidates of latent tuberculosis in exosomes from human serum

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15132
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Osada-Oka Mayuko 京都府立大学, 生命環境科学研究科, 准教授 (40347498)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 結核 / マクロファージ / エキソソーム / バイオマーカー

Outline of Final Research Achievements

The asymptomatic group, which is called latent infection of Mycobacterium tuberculosis (LITB), is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Thus, it has been required new diagnostic method to exactly establish LTBI. In this study, we examined the biomarker-targeted protein into macrophage-derived exosomes. After infection with active or latent Mycobacterium bovis Bacillus Calmette-Guerin, the expressions of both inflammatory and uninflammatory factor were up-regulated in RAW264.7 macrophages. Therefore, the protein were increased in exosomes from BCG-infected macrophages (BCG-exo) compared with exosomes from uninfected macrophages (none-exo). Moreover, it was identified 9 proteins, which is in BCG-exo derived from BCG-infected macrophages.

Free Research Field

生体防御機能学