2016 Fiscal Year Final Research Report
Genome analysis of Treponema pallidum and development of molecualar typing method
| Project/Area Number |
15K15137
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Ohnishi Makoto 国立感染症研究所, 細菌第一部, 部長 (10233214)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 梅毒 / Treponema pallidum / Molecular typing / genome analysis |
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| Outline of Final Research Achievements |
In this study 82 T. pallidum DNA-positive samples were obtained. Among them, 53 specimens could be typed, resulting 14 d/f (30/53, 56.6%) was shown to be the most frequent. Using GenomiPhi genomic DNA amplification reagent and SureSelect custom capture library for T. pallidum DNA enrichment system. SureSelect was construct by synthesizing RNA every 150 bp (75 bp overlapping) from the genome sequence information of T. pallidum Nichols strain. The error introduction by GenomiPhi was not found in this study. Thus, GenomiPhi amplification/SureSelect enrichment DNA were submitted to MiSeq. The coverage of the syphilis genome sequence from the 11 trial samples ranged from 0 to 1090. There were 4 specimens with coverage of 100 or less, 0, 16, 86, 97. Others showed coverage 211 ~ 1090, indicating that enough genomic information using GenomiPhi amplification and SureSelect enrichment.
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| Free Research Field |
細菌学
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