Screening for innate immune receptors recognizing allergic compounds

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15156
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Kagoshima University
• Principal Investigator: Hara Hiromitsu 鹿児島大学, 医歯学域医学系, 教授 (20392079)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: アレルギー / 免疫学 / 接触皮膚炎

Outline of Final Research Achievements

Since the activation of dendritic cells by allergic compounds (haptens) depends on the ITAM-Syk-CARD9 signal, we searched for myeloid ITAM-coupled receptors that recognize haptens. As a result, we found a plurality of ITAM coupled receptors showing binding properties to various haptens（TNCB、DNCB、oxazolon、FITC). Among them, focusing on two Ig superfamily receptors (called IgR2, IgR5) that associate with DAP12 and recognize multiple haptens, we investigated their ability of ITAM signal activation by using receptor-expressing NFAT-GFP reporter cells. As a result, both IgR2 and IgR5 activated the ITAM signal by hapten stimulation . Therefore, these two were identified as hapten receptors.

Free Research Field

免疫学