2016 Fiscal Year Final Research Report
Development of Diagnostic methods for detection of breast cancer using cell free DNA
| Project/Area Number |
15K15187
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Noguchi Emiko 筑波大学, 医学医療系, 教授 (40344882)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 血中遊離DNA |
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| Outline of Final Research Achievements |
The aim of this study is to investigate mutations of cell-free DNA (cfDNA) associated with breast cancer using next generation sequencer. We performed target sequences of TP53 and PIK3CA on 5 patients who were diagnosed with breast cancer. Mutations identified in these patients were also annotated with Catalogue Of Somatic Mutations In Cancer (COSMIC) database. We observed one somatic mutation at 7th exon of TP53 in several patients. In silico analysis revealed that this mutation is likely to influence on the function of TP53 protein.
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| Free Research Field |
遺伝学
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