Generation of antibodies that recognize cancer-associated core fucosylation in glycoproteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15201
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Okuda Tetsuya 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (20443179)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: コアフコース / モノクローナル抗体 / 糖鎖抗原 / がん / 糖タンパク質 / スフィンゴ糖脂質 / セラミド誘導体 / ワクチン

Outline of Final Research Achievements

To obtain monoclonal antibodies that recognize cancer-associated core fucosylation in glycoproteins, we examined application of our established immunity inducing method using artificial glycosphingolipid as immunogen. Particularly in this project, we have prepared two species of core fucosylated artificial glycosphingolipids and developed a screening method for obtaining the anti-core fucosylated glycoprotein antibody. On the other hand, we have obtained a monoclonal anti-glycosphingolipid IgG and found new activity of specific inhibitors for cellular β-hexosaminidases in this project.

Free Research Field

糖鎖工学、糖鎖免疫学