2016 Fiscal Year Final Research Report
Understanding of invasion/metastasis mechanism in colorectal cancer using organoid technology
Project/Area Number |
15K15297
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
|
Research Institution | Keio University |
Principal Investigator |
Sato Toshiro 慶應義塾大学, 医学部(信濃町), 准教授 (70365245)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | 大腸癌 / 浸潤 / 転移 / オルガノイド / 幹細胞 |
Outline of Final Research Achievements |
The pathological mechanism underlying cancer invasion and metastasis remains unknown. We generated 55 lines of human colon tumor organoids and comprehensively analyzed their molecular profiles, and thereby establishing functional assay platform for Genotype-Phenotype correlation. By applying CRISPR-Cas9 genome editing technology to organoids, we succeeded in prospective genetic engineering and gene function analysis system for human colorectal cancers. Our study provides research platform to understand pathological phenotype of cancers that confer them to invasion/metastasis capacity.
|
Free Research Field |
消化器内科学
|