2016 Fiscal Year Final Research Report
Development of an On-chip Simulator of Hepatic Lobule
| Project/Area Number |
15K15299
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Hiroshi 東海大学, 工学部, 准教授 (40533625)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | マイクロ流体デバイス / 肝細胞機能 / 肝小葉 |
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| Outline of Final Research Achievements |
During the drug development process, reproduction of the hepatic architecture and function in vitro is necessary and important to evaluate the efficacy and toxicity of the candidate drugs. In this study, we established two different types of oxygen gradient microfluidic device that mimics an in vitro hepatic lobular architecture.
After simulating the gradient of oxygen concentration, we evaluated the real oxygen concentration gradient in the devices using a sensing film coated with an oxygen-responsive dye. We also confirmed the survival and functions of hepatocytes that had been seeded inside the devices by using a live and dead cell staining kit and the indocyanine green uptake and excretion test, respectively.
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| Free Research Field |
消化器肝臓病学
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