Metabolomic studues on hypolactatemia and hypoketonema in hemodoalysis patients and on personalized medicine

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15326
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Tohoku University
• Principal Investigator: Sato Hiroshi 東北大学, 薬学研究科, 教授 (60215829)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 代謝応答 / 乳酸 / ケトン体 / メタボロミクス / 糖尿病 / ビオチン

Outline of Final Research Achievements

Metabolic responses during hemodialysis (HD) have studied by metabolomics of blood. Low molecules (lactate, pyruvate, alanine, 3-hydroxybutyrate (3-HB), and etc.) were measured by 1H NMR spectroscopy of dialysate, IRI (blood insulin) was quantified by the CLIA method. Plasma biotin and its metabolites were totally measured by the ELISA method. We developed a new selective method for determination of plasma biotin using UHPLC-MS/MS.
We found the group of patients in hemodialysis who had very low values of plasma lactate, pyruvate and alanine, with high values of plasma 3-HB. We have named these conditions as hypolactatemia and hyperketonemia, which often appeared in HD patients with diabetic mellitus. These metabolic profiling in the patients had correlation with plasma insulin removal during hemodialysis session. Biotin metabolites were revealed to accumulate in plasma of HD patients with cramp complications.

Free Research Field

腎臓内科