Molecular regulatory mechanism of podocyte, the filtration apparatus of glomeruli

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15330
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Okayama University
• Principal Investigator: Takei Kohji 岡山大学, 医歯薬学総合研究科, 教授 (40322226)
• Co-Investigator(Kenkyū-buntansha): 小川 大輔 岡山大学, 医歯薬学総合研究科, 准教授 (70535195)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ポドサイト / 糸球体 / ダイナミン / アクチン / アクチニン

Outline of Final Research Achievements

Podocyte is a cell that forms a glomerular filter for protein filtration. For the formation and the function of the glomerular filter, the cytoskeleton of podocyte needs to be appropriately regulated. However, it is poorly understood how cytoskeletons are regulated in podocyte. In this study, we aimed to elucidate regulatory mechanisms involved in the cytoskeletal regulation using two types of podocyte cell lines, Mouse Podocyte (MPC), and Human Podocyte (HPC). We found that dynamin 1 regulates microtubules, whereas dynamin 2 regulates actin cytoskeleton. Furthermore, we found that expression of dynamin 2 mutation found in Charcot Marie Tooth disease, a neurodegenerative disease, leads to aberrant actin cytoskeleton. These results strongly suggest that dynamin plays an essential role in the regulation of cytoskeleton in podocyte.

Free Research Field

細胞生物学