2016 Fiscal Year Final Research Report
Identification of novel glucagon-related peptides by using C-terminal anti-glucagon antibody
| Project/Area Number |
15K15345
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | グルカゴン |
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| Outline of Final Research Achievements |
We tried to identify novel glucagon-related peptides by using affinity chromatography with C-terminal anti-glucagon antibody and mouse plasma samples. However, due to insufficient sample volume, we failed to identify such molecules. Then we are now trying to do it using mouse pancreas or intestine. We also evaluated currently commercially available immunoassays for glucagon by comparing with our newly developed LC/MS/MS system and found that Mercodia sandwich ELISA has much higher correlation with LC/MS/MS compared to conventional RIA.
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| Free Research Field |
代謝学
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