2016 Fiscal Year Final Research Report
Identification of novel MSCs with hematopoiesis supporting capacity
| Project/Area Number |
15K15366
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 造血幹細胞 / 間葉系幹細胞 / ニッチ / 骨芽細胞 / 骨内膜 |
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| Outline of Final Research Achievements |
We found a subpopulation of osteoblast fraction (AL+) derived from endosteum by single cell gene expression analysis. This sub-fraction highly expressed hematopoietic niche factors and specifically expressed alpha8-integrin (Itga8). To clarify the characteristics of Itga8+AL+ cells, we compared gene expression profiles of Itga8+AL+ cells with known bone marrow mesenchymal stem cells and mesenchymal stem/progenitor cells derived from endocardium, and it was found that expression pattern of Itga8+AL+ cells was not included in either. Analysis of the in vitro culture system showed Itga8+AL+ cells had highly differentiation ability and proliferation potential. Although AL+ fraction has been recognized as a population of osteoblast, these results suggest that AL+ fraction contains mesenchymal stem/progenitor cells expressing Itga8 with different character from BM MSCs.
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| Free Research Field |
幹細胞生物学
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