Challenge to development of innovative method to control monocyte/macrophage lineages

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15386
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Morio Tomohiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30239628)
• Research Collaborator: OTSU Makoto 東京大学医科学研究所, 幹細胞プロセシング分野, 准教授
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 小児免疫 / アレルギー / 膠原病学

Outline of Final Research Achievements

We established a method to differentiate hematopoietic stem cells into monocytes using peripheral blood mononuclear cell derived iPS cells. We further refined a method to induce differentiation of monocytes to dendritic cells and also attempted to induce monocytes into M1 macrophages or to M2 macrophages. In this study, we have identified a novel responsible gene, OAS1, for B/Mo/DC deficiency with alveolar proteinosis through whole exome analysis of affected individuals. iPS cells were established from the patient; and a capacity of the cells to differentiate to monocytes or to DC was examined. In parallel, we generated OAS1 knock in mice to delve into the mechanism of monocyte differentiation defect. Finally, we were successful in inducing Aspergillus fumigatus-specific and Candida albicans-specific T cells using known overlapping peptides and optimized cytokines.

Free Research Field

小児科学、免疫学