2015 Fiscal Year Final Research Report
Investigation of pathogenesis of leukemia in Down syndrome patients using human ES and iPS cells
| Project/Area Number |
15K15402
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OTSU Makoto 東京大学, 医科学研究所, 准教授 (30361330)
EBIHARA Yasuhiro 東京大学, 医科学研究所, 特任准教授 (40302608)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Keywords | 遺伝・先天異常学 / iPS細胞 |
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| Outline of Final Research Achievements |
To clarify the mechanism causing hematological disorders and leukemia in DS patients, we employed induced pluripotent stem (iPS) cells derived from patients with DS and control iPS cells from healthy donor reprogrammed by the defined 3 or 4 factors (OCT3/4, KLF4, SOX2, and with or without c-MYC). We generated blood cells from DS and control iPS cells co-cultured with murine aorta-gonad-mesonephros-derivedstromal cell line. The harvested cells were analyzed for the presence of hematopoietic markers and the potentials of hematopoietic colony formation. Our results indicated that DS iPS cells could differentiate into identify the responsible genes for the acceleration of hematopoiesis in DS iPS cells, we carried out micro array analysis of hematopoietic cells derived from DS or control iPS cells. We then detected the high expression of 18 genes on chromosome 21 including RUNX1.
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| Free Research Field |
医歯薬学
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