2016 Fiscal Year Final Research Report

Development of non-human primate models to study neonatal HIE in regenerative medicine approach

Research Project

PDF

Project/Area Number	15K15404
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Embryonic/Neonatal medicine
Research Institution	Saitama Medical University
Principal Investigator	Yamanouchi hideo 埼玉医科大学, 医学部, 教授 (10250226)
Co-Investigator(Renkei-kenkyūsha)	SUGE Rie 埼玉医科大学, 医学部, 講師 (10342685) KATO Hidemasa 埼玉医科大学, 医学部, 准教授 (50292123)
Research Collaborator	KOMURA Makoto 埼玉医科大学, 医学部, 教授 (10422289) NISHIMURA Kunihiro 埼玉医科大学, 医学部, 研究員 OZAWA Shimpei 埼玉医科大学, 医学部, 研究員 YODA Mayuko 埼玉医科大学, 短期大学, 助教 KARINO Genta 埼玉医科大学, 医学部, 特別研究生 KOMURA Makoto 埼玉医科大学, 医学部, 教授 (50195468) KAKEI Hiroko 埼玉医科大学, 医学部, 助教 (20573877) SAKURAI Hayato 埼玉医科大学, 医学部, 助教 (40751938)
Project Period (FY)	2015-04-01 – 2017-03-31
Keywords	神経行動学 / 抗重力運動発達 / 補償生理基盤 / 低酸素暴露病態 / 霊長類モデル
Outline of Final Research Achievements	Neurologic sequelae of neonatal hypoxic ischemic encephalopathy (HIE) supposedly includes cerebral palsy, which is extrapyramidal symptoms due to occurrence of respiratory and circulatory insufficiency in the perinatal period. To study HIE preclinically, we aimed to develop the non-human primate model of common marmoset. A test system was designed and evaluated to simulate hypoxic environmentally exposed similarly to supposed situation with suppression of oxygen circulation under the other factors sufficient. Two primate groups were compared about the oxygen regulation to induce hypoxic and normal conditions. The results in the acute phase experiments with five as hypoxic and four as control groups suggested some "compensation-like" physical mechanism at blood oxygen saturation appropriately 20%. The acutely recovered infants from low oxygen exposure tended to view and behave differently in anti-gravity motion tests. This proposed model shows possibility for the furthermore studies.
Free Research Field	小児神経学