2017 Fiscal Year Final Research Report
Generation of hair shaft-specific Cre mice
| Project/Area Number |
15K15412
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Yamaguchi University (2016-2017)
Niigata University (2015) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 毛髪特異的 / Creマウス / Krt35 |
|---|
| Outline of Final Research Achievements |
In this project, we aimed to generate transgenic mice that specifically express Cre recombinase under the control by the promoter of a hair shaft-specific hair keratin Krt35 (Krt35-Cre). We initially made transgenic mice carrying the nucleotide sequences of Krt35-Cre. Subsequently, we backcrossed these mice with B6. 129X1-Gt(ROSA)26Sortm1 mice and successfully generated Krt35-Cre(+)/EYFP(+) mice. However, these mice did not specifically expressed EYFP in the hair shaft. Therefore we concluded that we could not achieve our objectives.
|
| Free Research Field |
皮膚科学分野
|
