2016 Fiscal Year Final Research Report
Identification of novel therapeutic target of melanoma with focus on gain-of-function mutation of SH3 domain of KIT
| Project/Area Number |
15K15414
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Fujita Health University (2016)
Nagoya University (2015) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOSHIKAWA Naohiko 神奈川県立がんセンター臨床研究所, がん生物学部, 部長 (70334282)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 悪性黒色腫 / KIT / SH3ドメイン |
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| Outline of Final Research Achievements |
The aims of this study are to identify novel theraputic targets of melanoma caused by KIT mutation, and to elucidate that a novel KIT mutation creates SH3 domain which binds to some proteins which have SH2 domain. Constitutive malignant KIT mutation expressed amelanotic melanocyte cell line, constitutive non-malignant KIT mutaion expressed amelonotic melanocyte cell line, and constitutive wild type KIT expressed amelonotic melonocyte cell line were established. The novel kit mutation was proven to create SH3 domain by proteome analysis. Some binding proteins which bound to the KIT mutation were identified. Moreover, some proteins, which overexpressed in constitutive malignant KIT mutation expressed amelonotic melonocyte cell line, were also identified.
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| Free Research Field |
皮膚科学
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