The development of newly radiosensitization based on dynamics on tumor hypoxic condition

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15442
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Southen Tohoku Research Institute for Neuroscience (2016-2017); Hirosaki University (2015)
• Principal Investigator: Takai Yoshihiro 一般財団法人脳神経疾患研究所, 南東北BNCT研究センター, センター長 (50107653)
• Co-Investigator(Kenkyū-buntansha): 廣瀬 勝己 一般財団法人脳神経疾患研究所, 南東北BNCT研究センター, 診療所長 (60623767) ; 佐藤 まり子 弘前大学, 医学部附属病院, 助教 (30645263)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 癌幹細胞 / 低酸素 / 放射線増感 / ホウ素中性子捕捉療法 / L-アミノ酸トランスポーター1 / Tirapazamine

Outline of Final Research Achievements

The hypoxic environment has changed responsiveness of tumors to radiotherapy in various ways. In this study, we examined whether control of tumor hypoxia contributes to sensitization of treatment. T98G and A172 glioblastoma cells survived under 1% hypoxia and continued to form spheroids in continuous culture for 2 weeks. Treatment of spheroids with hypoxic toxin tirapazamine (TPZ) at 40 μM significantly increased expression of L-amino acid transporter 1 (LAT 1), although no significant change was observed in cancer stem cell marker gene expression. LAT1 is involved in the uptake of drug BPA in boron neutron capture therapy (BNCT), suggesting the possibility of sensitizing BNCT by controlling hypoxia by TPZ.

Free Research Field

放射線腫瘍学