2016 Fiscal Year Final Research Report
Application of exosomal molecules as exposure dose assessment markers
| Project/Area Number |
15K15443
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MONZEN Satoru 弘前大学, 大学院保健学研究科, 講師 (20514136)
YOSHINO Hironori 弘前大学, 大学院保健学研究科, 助教 (10583734)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 被ばく医療 / マイクロRNA / 染色体異常解析 / 被ばくマーカー |
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| Outline of Final Research Achievements |
We performed array analysis of microRNA (miRNA) in serum obtained from 1-5 Gy X-ray whole-body irradiated mice and examined the relationship of miRNA expression after irradiation with dose and time. We identified one type of miRNA whose expression increased in a dose-dependent manner immediately after irradiation and 11 others whose expression decreased 24 h after irradiation. However, in the initial method employed for total RNA recovery, we used blood from several mice; as a result, we were unable to observe differences in miRNA expression among individual mice and obtain reproducible results. Therefore, we found a method that can extract and analyze high-quality RNA from individual mice. We are currently advancing miRNA expression analysis using an improved method. In addition, hierarchical clustering analysis based on array analysis of miRNA has revealed fluctuating expressions of genes associated with platelet function. Further investigation is underway.
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| Free Research Field |
放射線生物学
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