Acquiring radioresistance and cancer reprogramming after radiation to cancer cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15455
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Osaka University
• Principal Investigator: Okubo Hirofumi 大阪大学, 医学部附属病院, 医員 (50740263)
• Co-Investigator(Renkei-kenkyūsha): Ishii Hideshi 大阪大学, 大学院医学系研究科・癌創薬プロファイリング学, 特任教授(常勤) (10280736)
• Research Collaborator: Ogawa Kazuhiko 大阪大学, 大学院医学系研究科・放射線治療学, 教授 (40253984)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 放射線治療 / 治療抵抗性 / 癌幹細胞

Outline of Final Research Achievements

It is known from previous research results that cancer cells acquire resistance to radiation therapy by radiotherapy, but the mechanism of acquiring resistance to radiation therapy has not been elucidated. In this study, we started research under the hypothesis that radiation therapy would increase the proportion of cancer stem cells in the tumor and become resistant to treatment. Although it succeeded to create radiation resistant cell lines from several cancer cell lines, it took more than 1 year's unexpected period to prepare. Using them and proceeding with experiments, it turned out that cancer stem-like cells is increased in radioresistant cancer cells. Originally, we predicted that ZEB1 protein and microRNA-200 might be involved in reprogramming of cancer cells, but it was not necessarily the case. In the future, we plan to continue research to identify the key reprogramming factors.

Free Research Field

放射線生物学