Development of chip device to detect methylations on microRNAs

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15474
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: Nishida Naohiro 大阪大学, 医学系研究科, 助教 (50588118)
• Co-Investigator(Renkei-kenkyūsha): Mori Masaki 大阪大学, 大学院医学系研究科, 教授 (70190999) ; Obika Satoshi 大阪大学, 大学院薬学系研究科, 教授 (80243252)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 核酸 / 癌 / 遺伝子 / 臨床

Outline of Final Research Achievements

Chemical modifications of microRNA molecules potentially alter their biological functions. This study is aimed for a development of devices to comprehensively detect methylations of small nucleotide molecules including microRNAs. Efficient purifications of target microRNAs and highly accurate detection using mass spectrometry are key processes of this system. We have developed target enrichment method using affinity chromatography to precisely quantify chemical modifications on microRNAs from a very few amount of RNA samples. Furthermore, the optimizations of mass spectrometry settings including improvement of ionization efficiency and adjustment of measurement ranges enabled detection of a very small amount of chemical modifications (less than 5%) on microRNA oligonucleotides. To detect variety kinds of chemical modifications on many microRNA species, we have designed chip devices, on which oligonucleotides with complement DNA sequence of target microRNAs are densely printed.

Free Research Field

外科学