Analysis of post-transplant islet by organ transparency and macro three-dimensional image

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15481
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: National Center for Global Health and Medicine
• Principal Investigator: Shimoda Masayuki 国立研究開発法人国立国際医療研究センター, その他部局等, 膵島移植プロジェクト長 (40640529)
• Research Collaborator: MATSUMOTO Shinichi ; SHINOHARA Koya
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 臓器透明化 / 膵島移植

Outline of Final Research Achievements

Islet transplantation has attracted attention as a treatment for unstable type 1 diabetes. It is a less invasive cell transplantation therapy and it is already a standard treatment in some countries. On the other hand, one of the drawbacks of islet transplantation is a low survival rate. It is necessary to elucidate the cause, but it is a big obstacle not to be able to observe the implanted islets directly after the portal vein injection. Therefore, by using the transparency technique of organs and a sheet laser fluorescence microscope, the whole mouse liver was visualized and the pancreatic islets were observed after transplantation while maintaining the three-dimensional structure. As a result, it was possible to verify the overall distribution of the islets, decrease of the number of islets over time. The results were announced at the international conference.

Free Research Field

膵島移植