Development of biological diagnosis and treatment of pancreatic cancer using a new intracellular molecule

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15482
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: MORITA Naoki 国立研究開発法人産業技術総合研究所, 生命工学領域, 総括研究主幹 (60371085)
• Co-Investigator(Kenkyū-buntansha): 芳賀 早苗 北海道大学, 保健科学研究院, 特任講師 (60706505)
• Co-Investigator(Renkei-kenkyūsha): OKUDA Tetsuya 国立研究開発法人産業技術総合研究所, 生命工学領域生物プロセス研究部門, 主任研究員 (20443179) ; NODA Natsumi 北海道大学, 大学院保健科学研究院, 助教 (30624358)
• Research Collaborator: OZAKI Michitaka
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 膵癌 / 治療ターゲット分子 / 生物学的診断・治療法

Outline of Final Research Achievements

Evaluation of the biological significance of pXY molecule in pancreatic cancer cells revealed that pXY molecule was strongly involved in cell survival, and that pXY contributed to maintenance of intracellular antioxidant capacity and detoxification ability, and that reduced the easy-cytotoxicity by inhibiting FasL/Fas ligand. The secretion of pXY from the cells into the culture medium was detected by conventional ELISA method, whereas there remained a problem with the detectable limit concentration of pXY in blood by the present ELISA method. These results indicated that a novel antibody with higher sensitivity to pXY is required to achive the pXY detection in blood.

Free Research Field

分子生物学