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2016 Fiscal Year Final Research Report

Induction of smooth muscle cells from iPS cells derived from moyamoya disease patient

Research Project

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Project/Area Number 15K15520
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurosurgery
Research InstitutionHokkaido University

Principal Investigator

Houkin Kiyohiro  北海道大学, 大学病院, 教授 (90229146)

Project Period (FY) 2015-04-01 – 2017-03-31
Keywordsもやもや病 / iPS細胞 / 平滑筋細胞
Outline of Final Research Achievements

To investigate the pathogenesis of moyamoya disease, we induced smooth muscle cells from iPS cells derived from moyamoya disease patients. First, we induced neural crest cells from iPS cells. We found more than 99% of the cells were positive for p75. Next we differentiated these neural crest cells into smooth muscle cells, however, the cells become senescent during differentiation. Although surviving cells were almost positive for α-SMA and SM22, these cells could not be used for next analysis because of cell senescence. Improvement of the protocol is necessary to prevent cell senescence in the future.

Free Research Field

neurosurgery

URL: 

Published: 2018-03-22  

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