Novel in vivo optical imaging method monitoring oxidative stress

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15527
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Okayama University
• Principal Investigator: Abe Koji 岡山大学, 医歯薬学総合研究科, 教授 (20212540)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: in vivo イメージング / 脳梗塞 / Keap1 / Nrf2 / 酸化ストレスセンサー

Outline of Final Research Achievements

We investigated the time-dependent change of in vivo optical imaging of oxidative stress after stroke with OKD mice. The in vivo signals showed a peak at 1 d after tMCAO that was slightly correlated to infarct volume. The strong ex vivo signals, which were detected in the peri-ischemic area, corresponded to endogenous Nrf2 expression.
Our result of synchronization between in vivo imaging and endogenous Nrf2 expression might provide a benefit to verification of drugs targeting the Nrf2-mediated defense pathway and the therapeutic time window in acute phase of ischemic stroke.

Free Research Field

脳神経外科学