Investigation of regulatory role of osteoblast differentiation by long noncoding RNA

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15542
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Inose Hiroyuki 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (30615711)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 骨代謝 / ノンコーディングRNA / 骨芽細胞

Outline of Final Research Achievements

Recently, long non-coding RNAs (lncRNAs) have been shown to regulate various developmental and metabolic events. Osteoblast differentiation is an indispensable process in proper skeletal development and acquisition of bone mass, however, the physiological role of lncRNAs in osteoblast differentiation have not yet to be fully understood. Here, we found that CRNDE is an important regulator of this process through comprehensive analysis of lncRNAs expression during osteoblast differentiation by RNAseq. CRNDE was expressed in osteoblasts, and its expression increased over the course of osteoblast differentiation. Overexpression of CRNDE in osteoblasts promoted their differentiation, and on the opposite side, deletion of CRNDE expression inhibited osteoblast differentiation. Finally, we established CRNDE knockout mice using CRISPR/Cas9, and will continue to investigate its phenotype in further research. Our data shows that lncRNA is an important regulator of osteoblast differentiation.

Free Research Field

骨代謝