Functional analyses of mutant NLRP3 on the growth and differentiation of growth plate chondrocytes

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15549
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: Toguchida Junya 京都大学, ウイルス・再生医科学研究所, 教授 (40273502)
• Co-Investigator(Renkei-kenkyūsha): SHIMAZAKI Takeo 金沢医科大学, 総合医学研究所, 准教授 (50377420)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: CINCA症候群 / NLRP3 / 成長軟骨 / iPS細胞

Outline of Final Research Achievements

CINCA syndrome is one of auto-inflammatory diseases with a heterozygous mutation of NLRP3 gene and characterized as showing joint deformities along with symptoms related to chronic inflammation. To investigate the molecular mechanisms causing the abnormal growth plate, iPS cells with and without mutant NLRP3 gene were established and labeled with different markers, which can distinguish them. Chondrocytes were induced from each type of iPS cells and were cultured under the condition in which they shared culture supernatants. The results indicated that chondrocytes with mutant NLPR3 affected the phenotype of chondrocytes without the mutant gene by producing soluble factors.

Free Research Field

整形外科学、分子腫瘍学、幹細胞生物学