Development of the prevention method for steroid-induced osteonecrosis using screening system of cell-free technology for protein analyses

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15552
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Ehime University
• Principal Investigator: Imai Yuuki 愛媛大学, プロテオサイエンスセンター, 教授 (10423873)
• Co-Investigator(Renkei-kenkyūsha): SAWASAKI Tatsuya 愛媛大学, プロテオサイエンスセンター, 教授 (50314969)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 特発性大腿骨頭壊死 / ステロイド / グルココルチコイド

Outline of Final Research Achievements

To clarify the mechanism of the onset and progress of steroid-induced osteonecrosis of femoral head, we tried to identify the proteins interacting with Glucocorticoid Receptor (GR) using screening system of cell-free technology for protein analysis. As the results, we successfully identified more than 300 proteins, which interact with GR including not only reported proteins but also non-reported proteins relate to autophagy. This result indicates that steroid-induced osteonecrosis can be partly caused by protein degradation of non-genomic GR action as well as genomic actions.

Free Research Field

整形外科学