2016 Fiscal Year Final Research Report
Functional analysis of Arkadia in bone metabolism using innovative imaging technique and KO mice
| Project/Area Number |
15K15553
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | バイオテクノロジー / シグンル伝達 / 遺伝子 / タンパク質 / 細胞・組織 |
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| Outline of Final Research Achievements |
To identify the cofactor controlling Arkadia and to elucidate its molecular mechanism, screening of Arkadia binding protein was performed to obtain a candidate gene. Smad6 was identified as a new Arkadia target protein, Arkadia promotes differentiation by predominantly activating BMP signal during the late stage differentiation of osteoblasts, and ubiquitinates Smad6 which selectively suppresses BMP signal leading to degradation. A marked abnormality was found in the knockout mouse of E3 ubiquitin ligase Smurf 2 that negatively regulates the BMP signal prepared as a comparison target of Arkadia analysis.
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| Free Research Field |
骨代謝
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