2016 Fiscal Year Final Research Report
Development of a viral vector for gene therapy of chronic pain
| Project/Area Number |
15K15572
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 慢性疼痛 / 遺伝子治療 / 狂犬病ウイルス / G蛋白質 / 知覚神経指向性 / ウイルスベクター |
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| Outline of Final Research Achievements |
In this work, we assessed a lentiviral vector pseudotyped with the G protein of the rabies virus strain 1088, which prefers to infect sensory neurons, as a vector for gene therapy for chronic pain. We succeeded in production of infectious lentiviral particles with the G protein and found that an N-glycan addition on the G protein slightly enhanced the virus production. However, the virus production by the modified 1088 G protein was still significantly lower than that by the G protein of vesicular stomatitis virus. In order to assess using animal experiments, we consider that some improvements are needed to increase the production of the pseudotyped virus.
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| Free Research Field |
ウイルス学、獣医学
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