Hydrogen sulfide has a role as an endogenous relaxation factor in the bladder and prostate of male rats

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K15583
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kochi University
• Principal Investigator: Saito Motoaki 高知大学, 教育研究部医療学系基礎医学部門, 教授 (60273893)
• Co-Investigator(Kenkyū-buntansha): 清水 孝洋 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (00363276) ; 東 洋一郎 高知大学, 教育研究部医療学系基礎医学部門, 助教 (80380062) ; 清水 翔吾 高知大学, 教育研究部医療学系基礎医学部門, 助教 (90721853)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 硫化水素 / 膀胱 / 前立腺 / 弛緩

Outline of Final Research Achievements

We found that: (1) MPST and CAT were detected in the bladder and prostate and CBS was only detected in the prostate. On the other hand, CSE and DAO were not detected in both tissues. Immunoreactvities of these enzymes were mainly detected in the urothelium and smooth muscle layer of the bladder, and in the prostate glandular epithelium. (2) H2S was detected in the bladder and prostate tissues. (3) NaHS dose-dependently induced relaxation of pre-contracted BL-D, BL-T, PR-D and PR-V strips. There were no significant differences of the EC50 values or maximal relaxation rate against the pre-contractions among any of these tissues. Intravesically instilled GYY4137 significantly prolonged intercontraction intervals compared to the vehicle-treated group, and showed, although non-significant, tendency to reduce maximal voiding pressurecompared to the vehicle-treated group.

Free Research Field

下部尿路男性生殖器の薬理学