2016 Fiscal Year Final Research Report
DNA methylation analyses in the human placenta cells
| Project/Area Number |
15K15592
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 生殖医学 / エピジェネティクス |
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| Outline of Final Research Achievements |
Placental is common as the interface between mother and fetus to obtain nutrients essential for embryonic growth and gene expression is regulated by epigenetic mechanism. DNA methylation is a kind of epigenetic factors and globally reprogrammed after fertilization. Genomic imprinting is also leads to parent-of-origin specific mono-allelic expression of certain genes by DNA methylation (DMR). In this study, we performed genome-wide allelic DNA methylation analyses of human germ cells, blastocyst and purified trophoblast cells from placentas. We found that incomplete demethylation in btastocyst. RNA sequencing-based allelic expression analyses revealed that some of the placenta-specific maternal DMRs were associated with imprinted gene expression. These findings highlight the incomplete erasure of germline DNA methylation in the human placenta, which is important for understanding normal placental development and the pathogenesis of developmental disorders with imprinting effects.
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| Free Research Field |
産婦人科学、分子生物学
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