Getting genetic information from IVF media for noninvasive prenatal genetic screening

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15594
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Chiba University
• Principal Investigator: SHOZU Makio 千葉大学, 大学院医学研究院, 教授 (30226302)
• Research Collaborator: KOBAYASHI Tatsuya ; SONEHARA Hiroki ; TERAMOTO Syokichi ; SEGAWA Tomoya ; OOKUBO Tsuyoshi
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 産婦人科学 / 産科学 / 出生前診断 / 体外受精胚移殖 / 流産

Outline of Final Research Achievements

We examined what kind of genetic information are achievable from blastocyst culture media for possible non-invasive prenatal genetic screening. We found that serum hCG level within one week after implantation was inversely correlated with the number of X-chromosomes. We measured hCG level in used blastocyst culture medium and found that extremely high as well as low levels often resulted in non-implantation or abortion. Trace amount of genomic DNA was detected in the used medium, while mRNA was not. Significant amount of miRNA such as miR-22 as well as mitochondrial DNA (D-loop) were detectable in the blastocyst culture medium. Further study is warranted to determining relationship between these information and genetic constitution of embryo.

Free Research Field

生殖内分泌学