2015 Fiscal Year Final Research Report
Identification and functional analysis of Progesterone target gene in preterm birth
| Project/Area Number |
15K15597
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Yasushi 東京大学, 医学部附属病院, 講師 (40598653)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Keywords | 早産 / プロゲステロン / プロゲステロン受容体 / 脱落膜 / 子宮 / 標的遺伝子 |
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| Outline of Final Research Achievements |
Progesterone (P4) acts via the Progesterone receptor (PR). In pregnant women, P4 increases throughout pregnancy and remains high during labor and delivery. Given that levels of P4 do not decline before parturition, human parturition is involved in a functional PR withdrawal. It is speculated that preterm birth associated with the early decline of functional PR response, in the preterm uterus, the expression of PR target genes are decreased compared with normal delivery. To find the PR target genes related with preterm birth, we experimented as follows; (1) Using Ishikawa cells derived from human endometrium, Fifteen genes were identified as P4 responsive genes. Out of these 15 genes, we proposed that TRIM22 is a direct target gene of PR. (2) We cultured the decidual cells from normal and preterm delivery, the gene expression profile to response of P4 was analyzed by RNA-sequencing. As a result, the 385 genes were the specific genes in the decidua cells of preterm delivery.
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| Free Research Field |
生殖生理学
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