Creating eyeball bio-batteries and challenging to suppress local active oxygen

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15626
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: Abe Toshiaki 東北大学, 医学系研究科, 教授 (90191858)
• Co-Investigator(Renkei-kenkyūsha): Nishizawa Matsuhiko 東北大学, 大学院工学系研究科, 教授 (20273592)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 網膜色素上皮細胞 / マイクロ流路 / 活性酸素 / 低酸素 / ＶＥＧＦ

Outline of Final Research Achievements

We investigated the feasibility of the production of bio-batteries using micro-channel or eye ball by measuring active oxygen. It was possible to fabricate a porous polydimethylsiloxane (PDMS) film having an array of 10 micron pores and a PDMS mold having a flow path structure and culture retinal pigment epithelial cells (ARPE) in FY2015. In FY 2016, we could co-cultivate ARPE with HUVEC and found HUVEC migrate to the ARPE side, especially in some stress conditions. It turned out the possibility that mitochondria was involved in cultured cell death. Since the change in the generation of active oxygen was seen in mitochondria, we think there is a possibility to create the bio-battery, our final objective of production.

Free Research Field

眼科学