Implication of nerve growth factor in secondary lymphedema and pathogenesis

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K15653
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Nagasaki University
• Principal Investigator: AKITA Sadanori 長崎大学, 病院(医学系), 助教 (90315250)
• Co-Investigator(Kenkyū-buntansha): YOHIMOTO Hiroshi 長崎大学, 病院(医学系), 助教 (90513309)
• Co-Investigator(Renkei-kenkyūsha): MATSUSE Michiko 長崎大学, 原爆後障害医療研究所, 助教 (30533905) ; NAKAZAWA Yuka 長崎大学, 原爆後障害医療研究所, 助教 (00533902)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: ケロイド / 放射線 / 難治創傷 / 幹細胞

Outline of Final Research Achievements

Treatment of critical limb ischemia is sometimes difficult due to the patient’s condition. The hindlimbs of Sprague-Dawley rats, after 20 Gy X-ray radiation and surgical occlusion, were divided with superficial fascial flap, 5.0 × 106 adipose-derived stromal/stem cell (ASC) and both combined. There were no significant differences between the flap alone and ASC alone treatment groups. Wound healing was significantly increased in the combined treatment at 1 week and there was no wound by 2 weeks except for the no flap and no ASCs group. Von Willbrand factor, blood vessel, showed a significant increase in the combined treatment group, both at 1 week and 2 weeks. In the cell tracking, at 2 weeks, the GFP-tagged ASCs were significantly more positive in the no flap group than in the flap group. ASCs may be a potent cell source in radiated and occluded limbs by enhancing tissue blood flow and blood vessel density.

Free Research Field

形成外科