2017 Fiscal Year Final Research Report
Lineage tracing of adipose-derived stem/stromal cells in fat grafts.
| Project/Area Number |
15K15654
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Jichi Medical University |
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Principal Investigator |
Uda Hirokazu 自治医科大学, 医学部, 准教授 (20337306)
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| Co-Investigator(Kenkyū-buntansha) |
須永 中 自治医科大学, 医学部, 講師 (00406117)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 脂肪移植 |
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| Outline of Final Research Achievements |
We established Prx1Cre;Ai14 mice in which Prx1-expressing cells were genetically marked with tdTomato. Adipose-derived stem/stromal cells (ADSCs) from Prx1Cre;Ai14 mice expressed tdTomato. Using the transgenic mice, we transplanted fat grafts from wild mice to wild mice with tdTomato positive ADSCs, and analyzed the grafts with immunofluorescent imaging.
In the transplanted grafts, tdTomato-positive cells partially expressed perilipin or CD31, suggesting ADSCs contributed to regeneration of adipocytes and endothelial cells.
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| Free Research Field |
形成外科学
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