Development of a novel therapy for endotoxic shock using a humanized mouse model

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K15659
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: University of Tsukuba
• Principal Investigator: Shibuya Akira 筑波大学, 医学医療系, 教授 (80216027)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: ヒト化マウス / 敗血症 / CD300a

Outline of Final Research Achievements

We established a humanized mouse model, in which the immune responses in human endotoxic shock in vivo can be analyzed. By using this model, we examine the effect of anti-human CD300a monoclonal antibody on the endotoxic shock. First, we established a humanized mouse by transfer of human hematopoietic cells into severe immunodeficiency mouse (NOG mouse) and induced sepsis by cecal puncture and ligation. We analyzed survival, ALT, AST, and proinflammatory cytokines and chemokines such as IFNγ, TNF-a, IL-6, and IL-8 by ELISA and immune-beads analyses. These analyses suggest that anti-human CD300a monoclonal antibody is useful for the treatment of human endotoxic shock.

Free Research Field

免疫学