Development of novel method to estimate in vivo tissue injury using near infrared imaging system

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K15662
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Kyoto University
• Principal Investigator: Koike Kaoru 京都大学, 医学(系)研究科(研究院), 教授 (10267164)
• Co-Investigator(Kenkyū-buntansha): HIRAKAWA KEIKO 日本医科大学, 医学部, 助教 (30165162) ; KANAWAKU YOSHIMASA 日本医科大学, 医学部, 講師 (80465343) ; SATO NORIO 京都大学, 医学(系)研究科(研究院), 准教授 (30409205) ; SUZUKI TAKAO 京都大学, 医学(系)研究科(研究院), 研究員 (40328810)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: 近赤外 / 小腸 / 虚血 / 再灌流 / スペクトル / イメージング

Outline of Final Research Achievements

Near infrared (NIR) spectroscopy is a noninvasive, contactless and real-time analytical technology that may yield a variety of promising bedside monitoring systems in the field of emergency and critical care medicine. Intestinal ischemia/reperfusion (I/R) was conducted by 45 min of superior mesenteric artery occlusion followed by 4h of reperfusion in rats.
The reconstructed pictures produced by “in vivo” NIR imaging system demonstrated that the small intestine in the I/R 4h group was different from that in the before I group. Next we selected several regions of interest (ROIs) from each reconstructed picture, produced NIR spectrum for each ROI, and compared the temporal changes of NIR spectra. The NIR spectra obtained from the I/R 4h group showed apparently different pattern from those of the before and after I groups. No temporal change in NIR spectra was observed in the sham group. The NIR imaging system may become a novel tool to estimate tissue injury in vivo.

Free Research Field

救急医学